Laboratory Evaluation of Live Recombinant HVT - IBD Vaccine
نویسندگان
چکیده
Quality control tests were applied to prove the efficacy of the Vaxxitek HVT+IBD vaccine by Identity, sterility, titration, immunosuppression, safety and potency tests. Vaxxitek HVT+IBD vaccine proved to be safe when inoculated in 1-day old chicks free from maternal derived antibodies, broiler chicks and Swiss mice with 10field dose of the vaccine. The Vaxxitek HVT+IBD vaccine did not interfere with MDA and could be inoculated in 1-day broiler chicks safely and resulted in protective antibody response. Vaxxitek HVT+IBD vaccine was inoculated in 18-day old specific-pathogen-free embryonating chicken eggs (SPFECE) (3-days before hatching) without adverse effect on hatchability% and achieved protective response when challenged with vvIBDV compared with CEVA transmune vaccine which gave similar results. Vaxxitek HVT+IBD vaccine was stable after 10 blind passages on CEF cells that showed the same DNA band using specific primer for VP2 gene. Due to intra cellular nature of the vector vaccine so it gave long life protection as the immunity lasts till 9 month in our experiment after inoculation of the vaccine by one field dose to 1-day old broiler chicks then serum sample collected for serological tests (ELISA-SNT) and challenged with vvIBDV. [Rania Aly; El-Sanousi, A.and Susan, S. El-Mahdy. Laboratory Evaluation of Live Recombinant HVT-IBD Vaccine. Report and Opinion 2012;4(4):1-11]. (ISSN: 1553-9873). http://www.sciencepub.net/report. 1
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